Principles and Applications of Fluorescence Spectroscopy; Contents; 1 Absorption Spectroscopy Theory; 2 Determination of the CalcofluorWhite Molar Extinction Coefficient Value in the Absence and Presence of a1-Acid Glycoprotein; 3 Determination of Kinetic Parameters of Lactate Dehydrogenase; 4 Hydrolysis of p-Nitrophenyl-B-D-Galactoside with B-Galactosidase from E. coli; 5 Starch Hydrolysis by Amylase; 6 Determination of the pK of a Dye; 7 Fluorescence Spectroscopy Principles; 8 Effect of the Structure and the Environment of a Fluorophore on Its Absorption and Fluorescence Spectra.

9 Fluorophore Characterization and Importance in Biology10 Fluorescence Quenching; 11 Fluorescence Polarization; 12 Interaction Between Ethidium Bromide and DNA; 13 Lens culinaris Agglutinin: Dynamics and Binding Studies; 14 Förster Energy Transfer; 15 Binding of TNS on Bovine Serum Albumin at pH 3 and pH 7; 16 Comet Test for Environmental Genotoxicity Evaluation: A Fluorescence Microscopy Application; 17 Questions and Exe.